Vascular endothelial growth factor gene transfection improves angiogenesis in radioactive damaged masseter muscle

doi:10.3969/j.issn.2095-4344.2013.20.013

Abstract

Abstract:

BACKGROUND: Long-term high-dose radiation therapy is important to prevent the recurrence of cancer. But the radioactive rays can also make hurt to the normal tissues. So, researchers focus on the protection of the surrounding normal tissues in the radiotherapy.
OBJECTIVE: To study the impact of radiation on the rat masseter muscle and the revasularization ability of the irradiated tissue by vascular endothelial growth factor gene transfection.
METHODS: The Wistar rats were radiated by linear accelerator at a dose of 40 Gy. And then the irradiated muscle tissues were transfected with pcDNA4-HisMax-C/vascular endothelial growth factor 165 or empty plasmids. After 2 weeks of gene therapy, transforming growth factor β1 and vascular endothelial growth factor protein were determined using immunohistochemical staining, and the pathological change of the rat masseter muscles was observed under a light microscope.
RESULTS AND CONCLUSION: Transforming growth factor β1 protein was highly expressed in the irradiated tissue than the control tissue. Vascular endothelial growth factor protein was significantly increased in the gene transfected group than the radiation group, empty plasmid DNA transfection group and normal control group. These findings indicate that transforming growth factor β1 can improve damaged tissue repair, and vascular endothelial growth factor gene therapy can resume the ability of revasularization of irradiated tissue, thereby promoting irradiated tissue repair.

Key words: tissue construction, tissue construction and bioactive factor, irradiation injury, gene transfection, vascular endothelial growth factor, transforming growth factor beta 1, masseter muscle, immunohistochemistry, angiogenesis, other grants-supported paper

CLC Number:

R318

Yang Shu-fang, Zheng Jian-jin, Dong Gang, Li Tao, Wu Shuang-yan, Lu Shu-lai. Vascular endothelial growth factor gene transfection improves angiogenesis in radioactive damaged masseter muscle[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3699-3706.

Figures/Tables 6

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